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Summary
Spine loaches (Cobitidae) are common fishes in small rivers and creeks in Eurasia. We determined genome sequence of Cobitis taenia, denoted as tt_16D1C3L12 strain. We obtained Illumina paired-end and Nextera Long Mate-Pair libraries and sequenced them as 2x250bp long reads. Based on k-mer size analysis of trimmed and error-corrected reads the diploid genome appears to be around 1.267 Gbp. The homozygous loci were covered almost 40x and heterozygous loci have half-coverage (about 20x). The genomic sequence was assembled using abyss-2.0.2 into 320183 contigs (>=500bp) using solely paired-end read data and further scaffolded using Illumina long-mate pair reads into 195685 scaffolds (>=500bp). This work was also partially supported by The Ministry of Education, Youth and Sports from the Large Infrastructures for Research, Experimental Development and Innovations project "IT4Innovations National Supercomputing Center – LM2015070".
